StemCell Therapy

Abstract
Few options are available for treating patients with advanced prostate cancer (PC). As PC is a slow growing disease and accessible by ultrasound, gene therapy could provide a viable option for this neoplasm. Conditionally replication?competent adenoviruses (CRCAs) represent potentially useful reagents for treating prostate cancer (PC). We previously constructed a CRCA, Cancer Terminator Virus (CTV), which showed efficacy both in vitro and in vivo for PC. The CTV was generated on a serotype 5?background (Ad.5?CTV) with infectivity depending on Coxsackie?Adenovirus Receptors (CARs). CARs are frequently reduced in many tumor types, including PCs thereby limiting effective Ad?mediated therapy. Using serotype chimerism, a novel CTV (Ad.5/3?CTV) was created by replacing the ...

Source:
http://www.medworm.com/index.php?rid=7435952&cid=c_449_171_f&fid=33777&url=http%3A%2F%2Fonlinelibrary.wiley.com%2Fresolve%2Fdoi%3FDOI%3D10.1002%252Fjcp.24408

'Grinder' can listen to music player by wearing a loose wire coil around his neckDon't offer Rich Lee a pair of headphones to listen to music: he's already got a pair, even though you can't see them. They're implanted in his ears – a procedure carried out by a "body modification" expert.Now, by connecting his music player to a loose wire coil around his neck (which he can tuck under his shirt), Lee can listen to music without blocking out the outside world. The tiny magnets implanted invisibly in his outer ears pick up the signal and generate sound.But that's only the beginning. Lee, 34, who works as a salesman, intends to hook it up to an ultrasonic rangefinder – effectively giving himself bat-like echolocation. And he would like to have X-ray vision, super-strength, and anything else...

Source:
http://www.medworm.com/index.php?rid=7397462&cid=c_449_58_f&fid=36473&url=http%3A%2F%2Fwww.guardian.co.uk%2Ftechnology%2F2013%2Fjul%2F04%2Fheadphones-implanted-ear-grinder-rich-lee

'Grinder' can listen to music player by wearing a loose wire coil around his neckDon't offer Rich Lee a pair of headphones to listen to music: he's already got a pair, even though you can't see them. They're implanted in his ears – a procedure carried out by a "body modification" expert.Now, by connecting his music player to a loose wire coil around his neck (which he can tuck under his shirt), Lee can listen to music without blocking out the outside world. The tiny magnets implanted invisibly in his outer ears pick up the signal and generate sound.But that's only the beginning. Lee, 34, who works as a salesman, intends to hook it up to an ultrasonic rangefinder – effectively giving himself bat-like echolocation. And he would like to have X-ray vision, super-strength, and anything else...

Source:
http://www.medworm.com/index.php?rid=7397462&cid=c_449_58_f&fid=36473&url=http%3A%2F%2Fwww.guardian.co.uk%2Ftechnology%2F2013%2Fjul%2F04%2Fheadphones-implanted-ear-grinder-rich-lee

When Google CEO Larry Page blogged about his struggles speaking and, at times, breathing last week on his Google+ page he spotlighted a rare condition, bilateral vocal cord paralysis , which leaves sufferers short of breath and with few viable treatment options. This is likely to change in coming years. Page has deep pockets and has promised to fund research into the disorder via the Voice Health Institute . In the meantime scientists are experimenting with electrical stimulation technologies to enhance existing voice therapy as well as surgical treatments. [More]

Source:
http://rss.sciam.com/click.phdo?i=b3c657c36bd85f3126649923bb8701fe

When Google CEO Larry Page blogged about his struggles speaking and, at times, breathing last week on his Google+ page he spotlighted a rare condition, bilateral vocal cord paralysis , which leaves sufferers short of breath and with few viable treatment options. This is likely to change in coming years. Page has deep pockets and has promised to fund research into the disorder via the Voice Health Institute . In the meantime scientists are experimenting with electrical stimulation technologies to enhance existing voice therapy as well as surgical treatments. [More]

Source:
http://rss.sciam.com/click.phdo?i=b3c657c36bd85f3126649923bb8701fe

Authors: Yoshida T, Kondoh M, Mizuguchi H, Yagi K
Abstract
Hepatitis C virus (HCV) is a hepatotropic member of the Flaviviridae family and contains a 9.6 kb positive-sense RNA genome. Approximately 170-million people are infected with HCV worldwide. These people face increased risks of chronic hepatitis, cirrhosis and hepatocellular carcinoma compared with the general population. Transduction of the HCV genome into hepatocytes is essential for understanding the mode of action of HCV infection, and for preparing HCV, evaluating HCV replication, and screening anti-HCV drugs. Although electroporation of in vitro-synthesized HCV genome and transduction of plasmid vectors containing the HCV genome are widely used in HCV research, a more convenient system with higher transduction efficie...

Source:
http://www.medworm.com/index.php?rid=7281310&cid=c_449_13_f&fid=36240&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F23449406%3Fdopt%3DAbstract

Authors: Yoshida T, Kondoh M, Mizuguchi H, Yagi K
Abstract
Hepatitis C virus (HCV) is a hepatotropic member of the Flaviviridae family and contains a 9.6 kb positive-sense RNA genome. Approximately 170-million people are infected with HCV worldwide. These people face increased risks of chronic hepatitis, cirrhosis and hepatocellular carcinoma compared with the general population. Transduction of the HCV genome into hepatocytes is essential for understanding the mode of action of HCV infection, and for preparing HCV, evaluating HCV replication, and screening anti-HCV drugs. Although electroporation of in vitro-synthesized HCV genome and transduction of plasmid vectors containing the HCV genome are widely used in HCV research, a more convenient system with higher transduction efficie...

Source:
http://www.medworm.com/index.php?rid=7281310&cid=c_449_13_f&fid=36240&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F23449406%3Fdopt%3DAbstract

Bone Marrow Stem Cell Transplantation and Gene Therapy for Cystinosis
A presentation by Stephanie Cherqui, PhD, University of California, San Diego at the 2013 Day of Hope Cystinosis Research Foundation Family Conference, Balbo...

By: Natalieswish

Read this article:
Bone Marrow Stem Cell Transplantation and Gene Therapy for Cystinosis - Video

A child born to an HIV-infected mother in Mississippi may be cured after a swiftly administered course of drugs. A number of factors make the child’s case unique, however, and clinicians caution that we have not discovered a general cure for HIV yet. Still, the medical first may hint at ways to fight the AIDS-causing virus .

[More]

Source:
http://rss.sciam.com/click.phdo?i=95337d9798ea17db628f12536ca0a05b

Editor’s Note: Excerpted from Shouting Won’t Help: Why I--and 50 Million Other Americans--Can’t Hear You , by Katherine Bouton, published by Sarah Crichton Books, an imprint of Farrar, Straus and Giroux, LLC. Copyright © 2013 Katherine Bouton. All rights reserved.

[More]

Source:
http://rss.sciam.com/click.phdo?i=26ba9d76b5b91ce8e95b09bea6e3b5c4

A child born to an HIV-infected mother in Mississippi may be cured after a swiftly administered course of drugs. A number of factors make the child’s case unique, however, and clinicians caution that we have not discovered a general cure for HIV yet. Still, the medical first may hint at ways to fight the AIDS-causing virus .

[More]

Source:
http://rss.sciam.com/click.phdo?i=95337d9798ea17db628f12536ca0a05b

Editor’s Note: Excerpted from Shouting Won’t Help: Why I--and 50 Million Other Americans--Can’t Hear You , by Katherine Bouton, published by Sarah Crichton Books, an imprint of Farrar, Straus and Giroux, LLC. Copyright © 2013 Katherine Bouton. All rights reserved.

[More]

Source:
http://rss.sciam.com/click.phdo?i=26ba9d76b5b91ce8e95b09bea6e3b5c4

Muscle stiffness is a major clinical feature in Duchenne muscular dystrophy (DMD). DMD is the most common lethal inherited muscle-wasting disease in boys, and it is caused by the lack of the dystrophin protein. We recently showed that the extensor digitorum longus (EDL) muscle of mdx mice (a DMD mouse model) exhibits disease-associated muscle stiffness. Truncated micro- and mini-dystrophins are the leading candidates for DMD gene therapy. Unfortunately, it has never been clear whether these truncated genes can mitigate muscle stiffness. To address this question, we examined the passive properties of the EDL muscle in transgenic mdx mice that expressed a representative mini- or micro-gene (H2-R15, R2-15/R18-23/C, or R4-23/C). The passive properties were measured at the ages of 6 and 20 mo a...

Source:
http://www.medworm.com/index.php?rid=7067339&cid=c_449_68_f&fid=33708&url=http%3A%2F%2Fjap.physiology.org%2Fcgi%2Fcontent%2Fabstract%2F114%2F4%2F482%3Frss%3D1

If cupid had studied neuroscience, he’d know to aim his arrows at the brain rather than the heart. Recent research suggests that for love to last, it’s best he dip those arrows in oxytocin. Although scientists have long known that this hormone is essential for monogamous rodents to stay true to their mates, and that it makes humans more trusting toward one another, they are now finding that it is also crucial to how we form and maintain romantic relationships.

[More]

Source:
http://rss.sciam.com/click.phdo?i=1e2c8b293a6e7840f25387be9dac539e

Muscle stiffness is a major clinical feature in Duchenne muscular dystrophy (DMD). DMD is the most common lethal inherited muscle-wasting disease in boys, and it is caused by the lack of the dystrophin protein. We recently showed that the extensor digitorum longus (EDL) muscle of mdx mice (a DMD mouse model) exhibits disease-associated muscle stiffness. Truncated micro- and mini-dystrophins are the leading candidates for DMD gene therapy. Unfortunately, it has never been clear whether these truncated genes can mitigate muscle stiffness. To address this question, we examined the passive properties of the EDL muscle in transgenic mdx mice that expressed a representative mini- or micro-gene (H2-R15, R2-15/R18-23/C, or R4-23/C). The passive properties were measured at the ages of 6 and 20 mo a...

Source:
http://www.medworm.com/index.php?rid=7067339&cid=c_449_68_f&fid=33708&url=http%3A%2F%2Fjap.physiology.org%2Fcgi%2Fcontent%2Fabstract%2F114%2F4%2F482%3Frss%3D1

If cupid had studied neuroscience, he’d know to aim his arrows at the brain rather than the heart. Recent research suggests that for love to last, it’s best he dip those arrows in oxytocin. Although scientists have long known that this hormone is essential for monogamous rodents to stay true to their mates, and that it makes humans more trusting toward one another, they are now finding that it is also crucial to how we form and maintain romantic relationships.

[More]

Source:
http://rss.sciam.com/click.phdo?i=1e2c8b293a6e7840f25387be9dac539e

Potent growth-inhibitory effect of a dual cancer-specific oncolytic adenovirus expressing apoptin on prostate carcinoma.
Int J Oncol. 2013 Mar;42(3):1052-60
Authors: Zhang M, Wang J, Li C, Hu N, Wang K, Ji H, He D, Quan C, Li X, Jin N, Li Y
Abstract
Apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis in various human tumor cells, but not in normal cells. To explore the use of apoptin in tumor gene therapy, we assessed a recombinant adenovirus expressing the apoptin protein (Ad-hTERTp-E1a-Apoptin) in order to determine its lethal and growth-inhibitory effects on PC-3 and RM-1 cells in vitro and its antitumor effect on solid tumors in vivo....

Source:
http://www.medworm.com/index.php?rid=7005905&cid=c_449_6_f&fid=36721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2FPubMed%2F23338489%3Fdopt%3DAbstract

Authors: Kurosaki T, Uematsu M, Shimoda K, Suzuma K, Nakai M, Nakamura T, Kitahara T, Kitaoka T, Sasaki H
Abstract
In this experiment, we developed anionic ternary complexes for effective and safe ocular gene delivery. Ternary complexes were constructed by coating plasmid DNA (pDNA)/polyethylenimine (PEI) complex with anionic polymers such as ?-polyglutamic acid (?-PGA) and chondroitin sulfate (CS). The cationic pDNA/PEI complex showed high gene expression on the human retinal pigment epithelial cell line, ARPE-19 cells. The pDNA/PEI complexes, however, also showed high cytotoxicity on the cells and aggregated strongly in the vitreous body. On the other hand, the anionic ternary complexes showed high gene expression on ARPE-19 cells without such cytotoxicity and aggregation. Afte...

Source:
http://www.medworm.com/index.php?rid=7013349&cid=c_449_13_f&fid=32516&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2FPubMed%2F23302641%3Fdopt%3DAbstract

Authors: Kurosaki T, Uematsu M, Shimoda K, Suzuma K, Nakai M, Nakamura T, Kitahara T, Kitaoka T, Sasaki H
Abstract
In this experiment, we developed anionic ternary complexes for effective and safe ocular gene delivery. Ternary complexes were constructed by coating plasmid DNA (pDNA)/polyethylenimine (PEI) complex with anionic polymers such as ?-polyglutamic acid (?-PGA) and chondroitin sulfate (CS). The cationic pDNA/PEI complex showed high gene expression on the human retinal pigment epithelial cell line, ARPE-19 cells. The pDNA/PEI complexes, however, also showed high cytotoxicity on the cells and aggregated strongly in the vitreous body. On the other hand, the anionic ternary complexes showed high gene expression on ARPE-19 cells without such cytotoxicity and aggregation. Afte...

Source:
http://www.medworm.com/index.php?rid=7013349&cid=c_449_13_f&fid=32516&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2FPubMed%2F23302641%3Fdopt%3DAbstract

Potent growth-inhibitory effect of a dual cancer-specific oncolytic adenovirus expressing apoptin on prostate carcinoma.
Int J Oncol. 2013 Mar;42(3):1052-60
Authors: Zhang M, Wang J, Li C, Hu N, Wang K, Ji H, He D, Quan C, Li X, Jin N, Li Y
Abstract
Apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis in various human tumor cells, but not in normal cells. To explore the use of apoptin in tumor gene therapy, we assessed a recombinant adenovirus expressing the apoptin protein (Ad-hTERTp-E1a-Apoptin) in order to determine its lethal and growth-inhibitory effects on PC-3 and RM-1 cells in vitro and its antitumor effect on solid tumors in vivo....

Source:
http://www.medworm.com/index.php?rid=7005905&cid=c_449_6_f&fid=36721&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2FPubMed%2F23338489%3Fdopt%3DAbstract