StemCell Therapy

GMP Grade Stem Cell Cryopreservation Media

A Drug Master File for STEM-CELLBANKER has been registered with the Food and Drug Administration (FDA) (MF# 15785).

STEM-CELLBANKER is completely free of serum and animal derived components and contains only European or US Pharmacopoeia graded ingredients making is suitable for storage of cells developed for cell therapy applications. STEM-CELLBANKER is ready-to-use and requires no special devices, such as a controlled rate freezer, in order to achieve consistently high viabilities following resuscitation from cryopreservation, even over extended long-term storage.

STEM-CELLBANKER significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability after freeze-thaw.

Results of cryopreservation analysis performed at the Karolinska Institute on hESC and iPS cells with STEM-CELLBANKER shows significantly higher cell viability over conventional freezing medium while retaining cell pluripotency, normal karyotype and proliferation ability in HS293, HS306 and ChiPSA cell lines (Holm et al., Hum. Reprod. 2010).

Latest Publication:

Hata et al. (2014) Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells Nat Protoc. 2014 Oct;9(10):2354-68

Yamanaka S et al. (2014) A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific Reports 4 Article, number:359

Rodin et al. (2014) Monolayer culturing and cloning of human pluripotent stem cells on laminin-521based matrices under xeno-free and chemically defined conditions. Nat Protoc. 2014 Oct;9(10):2354-68

Miyamoto Y et al. (2012) Cryopreservation of Induced Pluripotent Stem Cells. Cell Medicine., Vol. 3, pp. 8995, 2012

Saliem M et al. (2012) Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution. World J Hepatol. May 27;4(5):176-83.

Holm et al. (2010) An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells. Hum Reprod. 25(5):1271-9. Epub 2010 Mar 5.

Nakamura A et al. (2010) Human primary cultured hepatic stellate cells can be cryopreserved. Med Mol Morphol. 43:107115

Cell Viability:

Survival of stem cells after thawing. (A) Growth rates of HS293, HS306 and CHiPS-A post-thawing for the first three passages. (B) The growth rates of both HS293 and HS306 frozen with STEM-CELLBANKER compared with cells frozen with 10% DMSO and FBS.

Cryopresevation of Mouse Kidney Tissue with STEM-CELLBANKER:

Mouse kidney was minced into about 5 mm square. The tissue samples were either immediately fixed with 10% formalin or cryopreserved with 1 ml of STEM-CELLBANKER at -80oC for one week. The cryopreserved samples were fixed with 10% formalin immediately after thawing. Paraffin sections of the both samples were stained with either H&E or anti-active caspase 3 antibody. Sign of necrosis or apoptosis was negligible in both of the fresh and cryopreserved tissue samples. Similar results were obtained in the cases of the tissue samples from a variety of mouse organs.

Preservation of mRNA after Cryopreservation of Cells with STEM-CELLBANKER:

Total RNA was extracted from the cells of a mouse lung fibroblast cell line either immediately after harvesting or after cryopreservation with STEM-CELLBANKER at -80oC for one week and thawing. mRNA levels in the two samples were compared by DNA microarray analysis, and the results showed that 93% of mRNAs was within the range of + 1.6-fold difference.

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Protocol:

STEM-CELLBANKER solutions are simple to use. Achieve the highest cell viability while maintaining stem cell pluripotency, normal karyotype and proliferation ability after cryopreservation using STEM-CELLBANKER.

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* AMSBIO is the global source for STEM-CELLBANKER reagents. STEM-CELLBANKER is a registered trade mark of and manufactured by NIPPON ZENYAKU KOGYO.

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Stem Cell Freezing Media STEM-CELLBANKER - amsbio

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