StemCell Therapy

With Stem Cells being used in the media and by other surgeons like an every day word, we want to clarify our standings on its use

Fat transfer, also known as fat grafting or autologous fat transfer, is the process of using the patients own fat to increase the volume of fat in another area of their body. The fat is harvested or extracted with a liposuction cannula. They are prepared for reintroduction into the body and injected into the new part of the body where the additional bulk is used for medical or cosmetic purposes. The most common donor areas, where fat is taken from, are the stomach, thighs, and waist.

The procedure is minimally invasive making it very appealing. Another advantage of fat transfer is that the body does not reject it because it is the patients own fat. However, there is a rate of absorption, a percentage of the transferred fat, that does not survive. Therefore, it is extremely important to select a surgeon that uses a proven technique with the highest fat survival rate.

Many women have turned to the Miami Breast Center looking for natural and permanent results. Dr. Khouri is a pioneer in the use of autologous fat to reconstruct and augment breasts. He is able to rebuild an entire breast using only the patients fat and the external tissue expander BRAVA. The BRAVA bra is worn a few weeks prior to surgery and stretches the tissue, both externally and internally, creating a matrix into which the transferred fat will be injected. This matrix is essential for fat placement and survival.

Dr. Khouri uses a very fine needle-like cannula, that he developed, to gently perform liposuction. The fat naturally contains stem cells, which are harvested in the process along with the fat and preserved in the transfer. However, nothing artificial is added nor biologically manipulated within the harvested fat.

Studies have shown that breasts rebuilt with this fat transfer process, pre-expanded with BRAVA, result in high fat survival and last forever. They look and feel natural and retain near-normal sensation. Compared to implants that on average need to be replaced every 10 years and can leave a woman with non-sensate breasts and nipples, Dr. Khouri offers a great alternative.

Growing concerns have emerged over the clinical practices and marketing claims made by the plastic surgery community promoting stem cell use in fat transfers. The term stem cells is hyped by the media and used by some surgeons like an everyday word. A small group of unethical practitioners are deliberately misleading and exploiting the public with false claims. Therefore, a task force was convened to address the false advertising claims that are not substantiated by scientific evidence.

The task force was made up of two of the worlds largest organizations of board-certified plastic surgeons, the American Society for Aesthetic Plastic Surgery (ASAPS) and the American Society of Plastic Surgeons (ASPS). They issued a joint position statement during The Aesthetic Meeting 2011, the annual meeting of ASAPS.

Dr. Roger Khouri himself sat on the task force and participated in the joint position statement. Here are a few of the recommendations made by the task force:

Dr. Khouri is a strong proponent of truth in advertising and very clearly states that his technique is a fat grafting procedure and not a stem cell procedure. Our technique of fat harvesting, preparation, and grafting preserves the native stem cells in the fat graft because we use very fine needle-like cannulas to gently liposuction the patients fat. The harvested fat is naturally rich in stem cells. Nothing artificial is added nor biologically manipulated within the harvested fat.

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Fat Transfer, Grafting to Breast using Stem Cells Truth ...

At the 2010 annual ASPS plastic surgery meeting in Toronto, I listened to Dr. Alexandra Cond Greens research presentation which concluded that 1) you can find quantities of stem cells using centrifugation techniques only without the use of collagenase, 2) the pellet at the bottom of the tube contains the highest % of stem cells and 3) stem cell enhanced fat grafting is better than regular fat grafting.

After she presented her paper, Dr. Peter Rubin, University of Pittsburgh Plastic Surgery Chief, (aka Mr. Stem Cell) said: Dr. Cond Green what you are proposing is quite provocative.

We believe that Dr. Rubin was referring to her findings that you can obtain stem cells by simply centrifuging the fat. This would be the golden minimal manipulation that could keep the FDA out of reach because these stem cells would not be classified as a drug! Dr. Cond Green, a plastic surgeon working with Dr. Pitanguys lab in Brazil, is onto something big here and I have invited her to share her results with you. Please see her comments below.

Figure 1

Autologous fat graft is considered nowadays the ideal soft-tissue filler because it is abundant, host compatible, and can be harvested easily and repeatedly. However many variations in long-term results of transplanted adipose tissue have been reported due to absorption and volume loss. Careful measures are employed to harvest, process and inject the fat in order to get the best possible intake. Still there is no consensus on the best method to prepare the fat and many controversies exist among these different steps.

Also, we have yet to know which part of the adipose tissue is more important for the graft take, the adipocytes, the other cells (stem cells, endothelial cells) or both.(Fig 1)

New evidence points to an improvement in vascularity and blood flow when stem cells are more concentrated in the fat to be transplanted. For therapeutic approaches, reproducible protocols of adipose-derived stem cells isolation and a large amount of cells are necessary, in order to respond to the increasing demand. Therefore, to practically understand what happens when adipose tissue is manipulated in the operating room, we studied the most common methods of fat processing employed by most plastic surgeons, in order to see which method yields the highest quantity of stem cells.

Under loco regional anesthesia, we aspirated adipose tissue from the inferior abdomen of healthy adult females undergoing liposuction with a blunt 3 mm cannula attached to a 10 ml Luer-lock syringe, using the superwet technique (at a rate of 1 ml of a solution containing normal saline with 1:1,000,000 of epinephrine per milliliter of aspirated tissue).

The lipoaspirates were separated into three samples that were processed by decantation (under the action of gravity in the syringe), washing with normal saline (in a ratio of 1:1 (vol/vol), 3 times) and centrifugation at 3,000 rpm/ 1,200g for 3 minutes.

Figure 2

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Centrifugation of Fat Tissue Yields More Stem Cells than ...

What is "Stem Cell Facelift"?

"Stem Cell Facelift" is not really an accurate description for a procedure where the patient's own fat is transplanted to the face in certain key areas to replace volume lost with aging. In many cases this is done with some type of facelift to address both volume loss and the effects of gravity. But the term stem cell facelift is only referring to the fat transfer to the face and therefore it should not be called a facelift.

What is fat grafting?

Sometimes also called fat transfer, autologous fat grafting, fat injections or fat grafting refers to the procedure of obtaining one's own fat from the abdomen or other parts of the body and re-implanting it with micro needles into the face, preferably within the deeper tissues of the face to obtain volume. As an injectable filler, your own fat is much better than a synthetic filler since it is your own tissue and lasts longer than any other filler.

What is longevity of fat grafting or injections?

Our doctor believes fat grafts persist longer than any other filler and does not involve any allergic reactions. With Our doctor's microinjection methods and with his careful harvesting techniques done at low pressures to preserve the membranes of the fat cells, 50 percent of the injected fat can last for 5 years. Our doctor has specialized methods to get the fat cells to preserve its integrity which is important for longevity. Our doctor points out that it is not the fat that is important but the fat cell for longevity of the transplanted graft to last.

Can fat transplantation to the face "so called stem cell facelift" be done as an isolated procedure without a traditional facelift?

Yes, if the problem is only volume loss, then autologous fat transplantation can be done alone. However, very often gravitational skin looseness in the neck and jowl also needs to be addressed with volume replenishment. Our doctor states the stem cell fat grafting and traditional facelifts are complementary and each address different components, ie. Volume loss and gravity.

What exactly is all the "hype" about stem cells?

Facial fat transfer procedures revolumize lost facial volume and give a very youthful effect but there is strong evidence that fat contains adult stem cells. Our doctor, a New York City facial plastic surgeon, believes that these stem cells may be responsible for a rejuvenating effect on the skin and other tissues of the face, other than just a volume filling effect from fat. Much research is currently being done about these extra benefits of stem cells and Our doctor is currently performing research on this topic with other worldwide researchers to determine its validity.

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Stem Cell Fat Grafting | Fat Transplantation

With a new document released today the FDA is more clearly on a path to regulate dubious stem cell clinics in the US.

There are more than 100 such American clinics that are selling stem cell treatments to patients and almost all of them use non-FDA approved stem cell products isolated from fat tissue.

The clinics have argued that they do not need FDA approval and just keep on raking inbig profits from vulnerable patients.

They have claimed no need for FDA approval because they believe that the stem cells isolated from fat tissue that they use are not drugs because they are not more than minimally manipulated. In English that means that the clinics are arguing that the purified fat stem cells are basically the same as overall fat tissue.

To me that doesnt make any sense.

A groundbreaking draft guidance statement today by the FDA for the first time sends the messagetothe clinics that the clinics are very likely wrong and could be subject to future regulatory action.

Itis important to point out that this FDA statement that mentions fat stem cells isdraft guidance meaning that it is not yet finalized, but make no mistake that this is the clearest snapshot to date on the FDAs views on fat stem cells and it is unlikely to fundamentally change during the comment period.

The bottom line is that fat stem cells are viewed by the FDA as drugs that must be vetted and approved prior to use by physicians and clinics.It also reinforces statements fromdraft guidance issued earlier in October that narrowed exceptions to the same-day surgical procedure guidance for use of biological materials such as stem cells.

In the new document today, the FDA even sets out isolation of fat stem cells as an example of more than minimal manipulation (emphasis mine):

Example 10-1: Original relevant characteristics of adipose tissue, a structural tissue, to pad and cushion against shocks generally include its bulk and lipid storage capacity. A manufacturer recovers adipose tissue by tumescent liposuction and processes the adipose tissue to isolate cellular components, commonly referred to as stromal vascular fraction, which is considered a potential source of adipose-derived stromal/stem cells. The HCT/P generally is considered more than minimally manipulated because the processing breaks down and eliminates the structural components that provide cushioning and support, thereby altering the original relevant characteristics of the HCT/P relating to its utility for reconstruction, repair, or replacement.

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Breaking: New FDA Draft Guidance Views Fat Stem Cells As ...

Fat Stem Cell Therapy 5 Step Treatment Procedure

Fat Stem Cell Therapy Procedure Avg. Rating: 5 out of 5 from 116 votes.

Autologous Adipose Enriched Stem Cells can help reverse and repair some of the world's most common medical diseases. Infinite Horizons Medical Center has perfected the Treatment and Recovery process to help patients suffering from some common medical conditions such as, Osteoarthritis, Pulmonary Disease, and Diabetes Type II, as well as some Cosmetic Procedures like Face Lifts, Breast Augmentation, and Anti-Aging. You can click on any procedure named above to get more detailed information.

Below you'll find our 5 step treatment procedure explained in detail:

Preparing the abdomen It all starts with a local anesthetic to the abdominal area.

Mini Liposuction A board certified cosmetic surgeon, who is also trained in liposuction techniques will then perform a manual mini liposuction procedure.

Fat Quantity The amount of fat that is extracted depends on which treatment we are performing. In most cases we only need between 40-200cc of fat.

Fat Distribution The surgeon will take it from both sides of the abdomen to ensure proper distribution.

Time Once the fat required has been harvested it will be handed to the Lab Tech to begin processing. The Fat Harvesting process has taken roughly 30-50 minutes.

Quantity of Fat Each test tube of harvested fat will now be handled by the Lab Tech who has been carefully trained by the Bio Tech Company that supplies us with this process. Not all the test tubes of fat will be processed. Some of the fat harvested may be needed to complete certain other cosmetic procedures.

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Procedure and Information on Fat Stem Cell Therapy

Mesenchymal stem cells, or MSCs, are multipotent stromal cells that can differentiate into a variety of cell types,[1] including: osteoblasts (bone cells),[2]chondrocytes (cartilage cells),[3]myocytes (muscle cells)[4] and adipocytes (fat cells). This phenomenon has been documented in specific cells and tissues in living animals and their counterparts growing in tissue culture.

While the terms mesenchymal stem cell and marrow stromal cell have been used interchangeably, neither term is sufficiently descriptive:

The youngest, most primitive MSCs can be obtained from the umbilical cord tissue, namely Wharton's jelly and the umbilical cord blood. However the MSCs are found in much higher concentration in the Whartons jelly compared to the umbilical cord blood, which is a rich source of hematopoietic stem cells. The umbilical cord is easily obtained after the birth of the newborn, is normally thrown away, and poses no risk for collection. The umbilical cord MSCs have more primitive properties than other adult MSCs obtained later in life, which might make them a useful source of MSCs for clinical applications.

An extremely rich source for mesenchymal stem cells is the developing tooth bud of the mandibular third molar. While considered multipotent, they may prove to be pluripotent. The stem cells eventually form enamel, dentin, blood vessels, dental pulp, and nervous tissues, including a minimum of 29 different unique end organs. Because of extreme ease in collection at 810 years of age before calcification, and minimal to no morbidity, they will probably constitute a major source for personal banking, research, and multiple therapies. These stem cells have been shown capable of producing hepatocytes.

Additionally, amniotic fluid has been shown to be a rich source of stem cells. As many as 1 in 100 cells collected during amniocentesis has been shown to be a pluripotent mesenchymal stem cell.[9]

Adipose tissue is one of the richest sources of MSCs. There are more than 500 times more stem cells in 1 gram of fat than in 1 gram of aspirated bone marrow. Adipose stem cells are actively being researched in clinical trials for treatment of a variety of diseases.

The presence of MSCs in peripheral blood has been controversial. However, a few groups have successfully isolated MSCs from human peripheral blood and been able to expand them in culture.[10] Australian company Cynata also claims the ability to mass-produce MSCs from induced pluripotent stem cells obtained from blood cells using the method of K. Hu et al.[11][12]

Mesenchymal stem cells are characterized morphologically by a small cell body with a few cell processes that are long and thin. The cell body contains a large, round nucleus with a prominent nucleolus, which is surrounded by finely dispersed chromatin particles, giving the nucleus a clear appearance. The remainder of the cell body contains a small amount of Golgi apparatus, rough endoplasmic reticulum, mitochondria, and polyribosomes. The cells, which are long and thin, are widely dispersed and the adjacent extracellular matrix is populated by a few reticular fibrils but is devoid of the other types of collagen fibrils.[13][14]

The International Society for Cellular Therapy (ISCT) has proposed a set of standards to define MSCs. A cell can be classified as an MSC if it shows plastic adherent properties under normal culture conditions and has a fibroblast-like morphology. In fact, some argue that MSCs and fibroblasts are functionally identical.[15] Furthermore, MSCs can undergo osteogenic, adipogenic and chondrogenic differentiation ex-vivo. The cultured MSCs also express on their surface CD73, CD90 and CD105, while lacking the expression of CD11b, CD14, CD19, CD34, CD45, CD79a and HLA-DR surface markers.[16]

MSCs have a great capacity for self-renewal while maintaining their multipotency. Beyond that, there is little that can be definitively said. The standard test to confirm multipotency is differentiation of the cells into osteoblasts, adipocytes, and chondrocytes as well as myocytes and neurons. MSCs have been seen to even differentiate into neuron-like cells,[17][18] but there is lingering doubt whether the MSC-derived neurons are functional.[19] The degree to which the culture will differentiate varies among individuals and how differentiation is induced, e.g., chemical vs. mechanical;[20] and it is not clear whether this variation is due to a different amount of "true" progenitor cells in the culture or variable differentiation capacities of individuals' progenitors. The capacity of cells to proliferate and differentiate is known to decrease with the age of the donor, as well as the time in culture. Likewise, whether this is due to a decrease in the number of MSCs or a change to the existing MSCs is not known.[citation needed]

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Mesenchymal stem cell - Wikipedia, the free encyclopedia

Lip Augmentation Using Fat Stem Cells

By: Regenix Clinics

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Lip Augmentation Using Fat Stem Cells - Video

Adipose vs Haemopoietic and Bone Marrow Sources
Fat vs other sources of stem cells? Fat is the foremost source of stem cells from practical, cost and clinical outcome perspective. Years of clinical practice and research show that treatment...

By: Global Medical Training Network [GMTN]

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Adipose vs Haemopoietic and Bone Marrow Sources - Video

Injecting Fat Stem Cells for Breast Augmentation

By: Global Medical Training Network [GMTN]

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Injecting Fat Stem Cells for Breast Augmentation - Video

fat stem cells 2
http://www.arthritistreatmentcenter.com Stem Cells | What about fat? Farshid Guilak and colleagues published an article in the journal Biorheology. In it the...

By: Nathan Wei

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fat stem cells 2 - Video

Stem Cell Injections in Knee May Improve Response to Medial Meniscectomy A Study Finds
Please like, subscribe, comment and share! ARTICLE http://jbjs.org/article.aspx?articleid=1809954 The objectives were to evaluate the safety of intra-articul...

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Stem Cell Injections in Knee May Improve Response to Medial Meniscectomy A Study Finds - Video

U Autologous Stem Cell Skin Care for Anti Aging - American Cryostem
http://tinyurl.com/q2egdra - With U Autologous stem cell skin care, along with adipose tissue storage, patients can now use their own fat stem cells for anti...

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U Autologous Stem Cell Skin Care for Anti Aging - American Cryostem - Video

Fat stem cells regenerate cartilage in osteoarthritis
http://www.stemcellsarthritistreatment.com http://www.arthritistreatmentcenter.com Dr. Bui Hong Thien Khanh, head of orthopedics at the Ho Chi Minh City Medi...

By: Nathan Wei

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Fat stem cells regenerate cartilage in osteoarthritis - Video

Wow! Another source of fat stem cells
http://www.stemcellsarthritistreatment.com A recent study by Koh and colleagues published in the journal Arthroscopy highlighted another source of stem cells...

By: Nathan Wei

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Wow! Another source of fat stem cells - Video

ADIPOSE FAT STEM CELLS - SVF Now Available in Texas - Innovations Medical Stem Cell Center
http://www.InnovationsStemCellCenter.com 214.699.6948.

By: InnovationsStemCell

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ADIPOSE FAT STEM CELLS - SVF Now Available in Texas - Innovations Medical Stem Cell Center - Video

JUPITER, Fla., Nov. 5,2012 /PRNewswire/ --BioRestorative Therapies, Inc. ("BRT" or the "Company") (OTCBB: BRTX),a life sciences company focused on stem cell based cellular therapies for various personal medical applications, announced todaythat its common stock hasbegun trading on theOTC Bulletin Board.

Mark Weinreb, Chief Executive Officer of BRT, commented on the uplisting, "We went through a very thorough and diligent review process by FINRA to attain the Bulletin Board listing. The Bulletin Board trading platform allows for more potential investors interested in purchasing our stock.We are extremely pleased to provide investors increased visibility and transparency that this regulated quotation service provides so the Company can continue the pursuitof itsgoal of becoming a leader in developing ground-breaking medical procedures."

About BioRestorative Therapies, Inc.

BioRestorative Therapies, Inc.("BRT") developsmedical procedures using cell and tissue protocols, primarily involving adult stem cells (non-embryonic), and allowing patients to undergo minimally invasive cellular-based treatments.BRT is developing the following scientific initiatives:OurbrtxDISCProgram (DiscImplanted StemCells)offers a non-surgical treatment for bulging and herniated discs andaddresses the gap between non-invasive and invasive back procedures.

OurThermoStemProgramfocuses on treatments for metabolic disorders (diabetes, heart disease, etc.) and obesity anduses brown fat stem cells. Initial research indicates that increased amounts of brown fat in the body may be responsible for additional caloric burning as well as reduced glucose and lipid levels in the body. The Company also offers plant stem cell-based facial creams and products under theStem Pearlsbrand atwww.stempearls.com.

This press release contains "forward-looking statements" within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of 1934, as amended, and such forward-looking statements are made pursuant to the safe harbor provisions of the Private Securities Litigation Reform Act of 1995. You are cautioned that such statements are subject to a multitude of risks and uncertainties that could cause future circumstances, events or results to differ materially from those projected in the forward-looking statements as a result of various factors and other risks, including those set forth in the Company's Form 10-K filed with the Securities and Exchange Commission. You should consider these factors in evaluating the forward-looking statements included herein, and not place undue reliance on such statements. The forward-looking statements in this release are made as of the date hereof and the Company undertakes no obligation to update such statements.

Investor Contacts: KCSA Strategic Communications Philip Carlson / Josh Dver +1 212.896.1233/+1 212.896.1239 pcarlson@kcsa.com / jdver@kcsa.com

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BioRestorative Therapies Announces Stock Trading On OTC Bulletin Board

A holistic approach: Part 2: Anti-Aging Treatment with Patient-Derived Fat Stem Cells; Part 2
Part 2 of a presentation done by Dr. Richard DeAndrea. He presents on Adistem Technology at the A4M conference in Bangkok, Thailand. Patient derived adipose stem cell extraction, isolation, activation and treatment are discussed. He also review Anti-aging effects and medical case studies using autologous stem cell therapyFrom:adistemViews:528 2ratingsTime:14:28More inScience Technology

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A holistic approach: Part 2: Anti-Aging Treatment with Patient-Derived Fat Stem Cells; Part 2 - Video

Alan discusses his stem cell treatment by Dr Harry Adelson for arthritis of the knees
Alan discusses his success using bone marrow and fat stem cells in his arthritis knees. He reports 80% improvement in his pain and activities. Dr Harry Adelson has been performing stem cell procedures for orthopedic pain since February 2010 and has had hundreds of successes.From:Harry AdelsonViews:104 0ratingsTime:01:50More inScience Technology

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Alan discusses his stem cell treatment by Dr Harry Adelson for arthritis of the knees - Video

Storing Fat Stem Cells with Dr. Jeffrey Ptak
Dr. Ptak talks to CBS Channel 5 in Arizona about storing fat stem cells and how that can help adults. Stem cell therapeutics can help people fight all sorts of diseases.From:drjptakViews:177 2ratingsTime:03:31More inEducation

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Storing Fat Stem Cells with Dr. Jeffrey Ptak - Video

Washington, Oct 16 (IANS) US scientists have discovered a link between expanding waistlines and certain cancers, saying that excess fat is a key factor in the progression of the disease.

Although diet is an important factor, the direct effect of excess fat tissue on tumours has to be considered, said Mikhail Kolonin, senior study author and associate professor of Regenerative Medicine at the Brown Foundation Institute, University of Texas.

"For the first time, we have demonstrated that excess fat is a key factor in cancer progression regardless of the diet contributing to the extra weight," Kolonin said, the journal Cancer Research reports.

The World Health Organisation reports that in 2008 there were more than 1.4 billion obese adults worldwide, with 7.6 million succumbing to cancer that year, according to a Texas statement.

Tumours emit a signal that attracts progenitor cells from white adipose (fat) tissue in mouse models of cancer. These cells in turn support the network of blood vessels that nourish tumours - a process called tumour angiogenesis.

Like a stem cell, a progenitor cell can differentiate into a specific type of cell. Stem cells can replicate indefinitely, but progenitor cells can divide only a limited number of times.

"Our experiments show that fat progenitors are recruited by tumours, where they incorporate into blood vessels and become fat cells," said Yan Zhang, who led the study as research scientist at the UT Health Medical School.

"The next step in this research would be to inactivate fat progenitor cells in an effort to slow cancer progression," said Kolonin.

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'Expanding waistlines linked with cancers'